Workshop: PAM fluorometry for ecologists

Chlorophyll a fluorescence measurements are powerful to describe fundamental processes  associated with photosynthesis, and are increasingly being used in investigations of the ecophysiological and ecological performance of photosynthetic marine organisms.

ITRS delegates and local researchers have the opportunity to attend a 2-day workshop on measuring photosynthesis and the application of Pulse Amplitude Modulated (PAM) Fluorometry.

The workshop will be held at UWA on 20-21 January. It will focus on methodological aspects of measuring photosynthesis including both traditional methods and chlorophyll fluorescence and the use of PAM Fluorometry in marine ecology. The workshop will take the format of an intensive learning course in 3 modules, and will combine theory, laboratory and field exercises. Download the workshop outline here.

The workshop will be aimed at beginner to intermediate users of chlorophyll fluorescence analysis techniques and will cover the theory of chlorophyll fluorescence analysis, correct use of a range of PAM fluorometers as well as data interpretation in the context of the wider plant/alga metabolism. A prime focus across the 2 days will be improving interpretation of chl-a fluorescence data in the context of the wider metabolic framework

Diver measuring chlorophyll fluorescence of Australian kelp, using the Diving Pam from Walz (Photo: T. Wernberg).

Participants will not only get the opportunity to use the Diving- and Mini-PAM fluorometers (Walz GmbH),  but will get an opportunity to see and use the latest equipment from the Australian company Aquation Pty. Ltd., including their submersible Shutter Fluorometer, submersible Photosynthesis-Respiration System and absorptance meter.

The workshop is organised jointly by Murdoch UniversityAquation Pty. Ltd. and the University of Western Australia. It will be led by Dr John Runcie (Honorary Research Associate at the University of Sydney and Director of Aquation) and Dr Jeff Cosgrove (Murdoch University).

Dr John Runcie was awarded his PhD from the University of Sydney in 2001 examining the nutrient uptake kinetics of marine macroalgae.  Since then he has undertaken several research posts (Hawaii Institute of Marine Biology, Australian Antarctic Division) examining the applications of modulated fluorescence techniques in marine pollution studies.  John currently owns and operates a small business Aquation Pty. Ltd. designing, manufacturing and selling submersible monitoring equipment including modulated fluorometers.

Dr Jeff Cosgrove was awarded his PhD after researching the interrelationship between Chl fluorescence, O2-evolution and C-fiixation estimates of primary production and the use of Chl fluorescence to investigate microalgal physiological responses to environmental conditions. Jeff is currently adjunct lecturer at Murdoch University and a Senior Environmental Officer at the Swan River Trust.

Participation costs $170 (incl GST) and includes both days and all activities. Numbers are limited to 20 participants to maintain an interactive learning environment so register fast to ensure a spot. Please register your intent to participate here.